Gel Permeation Chromatography (GPC) is a separation method for the determination of molecular weight averages (M_n) and molecular weight distribution (PDI=M_w/M_n). The testing report can include calibration plot, chromatogram, number average molecular weight and PDI of the polymer. GPC-chloroform is using chloroform as a solvent. Gel Permeation Chromatography (GPC) separates molecules on the basis of hydrodynamic size (not M_w). The large molecules move more rapidly through the GPC column, and in this way the mixture can be separated. The elution volume of a molecule is related to its hydrodynamic volume. To relate the elution volume to the molecular weight of a polymer, a calibration with molecular weight standards is needed.

Equipment
Pump: Waters Alliance HPLC System, 2690 Separation Module.
Two columns: Agilent, PLGEL 5μm, MIXED-D, 300x7.5 mm.
Detectors:
Waters 2410 Differential Refractometer (RI)
Waters 2998 Photodiode Array Detector (PDA)
Solvent: Chloroform with 0.25%  of TEA
Flow Rate: 1 mL/min
Injection: 100 μL.